Kafukufuku wathu akuwonetsa kuti Ganoderma lucidum/reishi/lingzhi amawonetsa antitumor pama cell a osteosarcoma mu vitro.Zinapezeka kuti Ganoderma lucidum imalepheretsa kukula kwa maselo a khansa ya m'mawere ndi kusamuka mwa kupondereza chizindikiro cha Wnt / β-catenin.Imaletsa khansa ya m'mapapo chifukwa cha kusokonezeka kwa zomatira komanso kuchititsa kuwonongeka kwa MDM2-mediated Slug.Ganoderma lucidum imaletsa khansa ya m'mawere pochepetsa njira ya PI3K/AKT/mTOR, Ganoderma lucidum imagwira ntchito yoletsa kutupa m'maselo owopsa a leukemia potsekereza njira ya MAPK.
CCK-8 ndi kuyesa kwa mapangidwe a koloni, poyesa zotsatira za Ganoderma lucidum pa osteosarcoma cell line viability ndi kuchulukana, adawonetsa kuti Ganoderma lucidum imachepetsa kuchuluka kwa ma cell a MG63 ndi U2-OS munjira yodalira nthawi ndi ndende, ndikuchepetsa kuthekera kwa ma cell kupanga koloni.
Ganoderma lucidum imayang'anira kufotokozera kwa majini a proapoptotic, ndipo kusanthula kwa cytometry kunasonyeza kuti apoptosis ya MG63 ndi maselo a U2-OS amawonjezeka pambuyo pa chithandizo ndi Ganoderma lucidum.Kusuntha kwa ma cell ndi maziko a machitidwe osiyanasiyana achilengedwe, kuphatikiza angiogenesis, machiritso a bala, kutupa, ndi metastasis ya khansa.Ganoderma lucidum imachepetsa kusamuka ndi kuwukira kwa ma cell onse ndikuletsa kuchulukana, kusamuka, ndi kuwukira, ndipo imapangitsa apoptosis ya ma cell a osteosarcoma.
Chizindikiro cha Aberrant Wnt / β-catenin chimagwirizana kwambiri ndi mapangidwe, metastasis, ndi apoptosis ya mitundu yambiri ya khansa, ndi kuwongolera kwa chizindikiro cha Wnt / β-catenin chikuwonetsedwa mu osteosarcoma.
Mu kafukufukuyu, kafukufuku wa atolankhani awiri a luciferase adawonetsa kuti chithandizo cha Ganoderma lucidum chimalepheretsa CHIR-99021-activated Wnt/β-catenin signing.Izi zikutsimikiziridwa ndi kuwonetsera kwathu kuti kulembedwa kwa ma jini a Wnt target, monga LRP5, β-catenin, cyclin D1, ndi MMP-9, amaletsedwa pamene maselo a osteosarcoma amathandizidwa ndi Ganoderma lucidum.
Kafukufuku wam'mbuyomu awonetsa mu zitsanzo zachipatala kuti LRP5 imayendetsedwa ndi osteosarcoma yokhudzana ndi minofu yokhazikika, komanso kufotokozera kwa LRP5 kumagwirizana ndi matenda a metastatic komanso kupulumuka kopanda matenda, zomwe zimapangitsa LRP5 kukhala chandamale chochiritsira cha osteosarcoma.
β-catenin yokha ndiyo cholinga chachikulu mu njira yowonetsera Wnt / β-catenin, ndipo kufotokozera kwa β-catenin mu osteosarcoma kumawonjezeka kwambiri.Pamene β-catenin imalowa mu nyukiliyasi kuchokera ku cytoplasm, imayambitsa kufotokoza kwa majini ake omwe amatsata pansi, omwe amaphatikizapo cyclin D1, C-Myc, ndi MMPs.
Myc ndi imodzi mwazinthu zazikulu za proto-oncogenes ndipo imagwira ntchito yofunika kwambiri pakuwongolera kutsegulira, kulembedwa, ndi kuletsa kwa jini. osteosarcoma.
Cyclin D1 ndi gawo lofunikira la cell cycle G1 ndikufulumizitsa kusintha kwa gawo la G1/S.Kuchulukirachulukira kwa cyclin D1 kumatha kufupikitsa kuzungulira kwa ma cell ndikulimbikitsa kuchuluka kwa ma cell mumitundu yosiyanasiyana ya chotupa.
MMP-2 ndi MMP-9 ndi ma stromelysins omwe amatha kusokoneza zigawo za extracellular matrix, chinthu chofunikira kwambiri pa chotupa cha angiogenesis ndi kuwukira.
Izi zikusonyeza kuti jini ya Wnt/β-catenin imathandizira kwambiri pakukula kwa osteosarcoma, komanso kuti kutsekereza ma node azizindikirozi kumatha kukhala ndi chithandizo chodabwitsa.
Pambuyo pake, tidazindikira mafotokozedwe a mRNA ndi mapuloteni amtundu wa Wnt/β-catenin wokhudzana ndi ma sign ndi PCR ndi kumadzulo blotting.M'maselo onse awiri, Ganoderma lucidum inaletsa kufotokoza kwa mapuloteni ndi majini.Zotsatirazi zikuwonetsanso kuti Ganoderma lucidum imalepheretsa chizindikiro cha Wnt/β-catenin potsata LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, ndi MMP-9.
E-cadherin ndi transmembrane glycoprotein yomwe imawonetsedwa kwambiri m'maselo a epithelial ndipo imayimira kumamatira pakati pa ma cell a epithelial ndi ma cell a stromal.Kuchotsa kapena kutayika kwa mawu a E-cadherin kumabweretsa kutayika kapena kufowoka kwa kumamatira pakati pa maselo otupa, zomwe zimapangitsa kuti maselo otupa azitha kuyenda mosavuta, ndiyeno kupangitsa chotupacho kulowa, kufalikira, ndikufalikira.Mu phunziro ili, tapeza kuti Ganoderma lucidum ikhoza kukweza E-cadherin, motero imatsutsana ndi phenotype ya Wnt / β-catenin-mediated ya osteosarcoma maselo.
Pomaliza, zotsatira zathu zikuwonetsa kuti Ganoderma lucidum imatchinga chizindikiro cha osteosarcoma Wnt/β-catenin ndipo pamapeto pake imabweretsa kuchepa kwa ma cell a osteosarcoma.Zomwe zapezazi zikuwonetsa kuti Ganoderma lucidum ikhoza kukhala chithandizo chothandizira komanso chothandiza pochiza osteosarcoma, zinthu zofananira zomwe zikuphatikiza.ganoderma lucidum spore mafuta softgels/reishi spore oil softgels,ganoderma lucidum spore powder/reishi spore powder
Nthawi yotumiza: Apr-18-2022